Year: 2018 | Month: February | Volume 8 | Issue 1
Discrimination of Beta-lactam Resistant Escherichia coli isolated from Poultry and Poultry Farm Workers using (GTG) 5-Rep-PCR
Abstract:
The study was aimed at examining the discrimination of beta-lactam resistant E. coli isolated from poultry and poultry farm workers in Andhra Pradesh (India). Faecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for beta-lactam resistance phenotypically, by disc diffusion method and genotypically, by PCR targeting blaCTX-M group-1 and 2 beta-lactamase genes. Beta-lactam resistant E. coli were serotyped based on their ‘O” antigen and then genotyped using (GTG) 5 repetitive sequence based PCR (Rep-PCR). Out of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) recovered, 12 isolates were found to be beta-lactam resistant, of which seven carried extended-spectrum beta-lactamase (ESBL) phenotype. All the phenotypically resistant isolates carried multiple beta-lactamase genes. Serological typing of the 12 resistant E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT and Rough). Rep-PCR fingerprinting allowed the discrimination of 12 resistant E. coli isolates into 11 Rep-PCR genotypes. The numerical index of discrimination was 0.999. Present findings enabled the observation of genetic diversity between beta-lactam resistant E. coli isolated from healthy poultry and poultry farm workers in Andhra Pradesh (India) based on their Rep-PCR genotype.
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